Journal: Arthritis Research & Therapy

Article Title: Heavy metal exposure reverses genetic resistance to Chlamydia -induced arthritis

doi: 10.1186/ar2610

Figure Lengend Snippet: The effect of prior exposure to mercuric chloride (HgCl 2 ) on serum anti- Chlamydia antibodies and anti-collagen antibodies in Chlamydia -induced arthritis. (a) HgCl 2 -exposed rats had significantly higher serum IgG1 anti- Chlamydia antibodies than non-exposed control rats ( P < 0.001). Control rats had slightly higher IgG2a anti- Chlamydia antibodies than the HgCl 2 -exposed rats ( P = 0.12). (b) HgCl 2 -exposed Brown Norway (BN) rats had higher levels of IgG antibodies to rat type II collagen than controls ( P < 0.01) (n = 8 in each group). O.D., optical density.

Article Snippet: The ELISA kit for the rat anti-type II collagen antibodies was from Chondrex (Redmond, WA, USA) and followed the protocol of the manufacturer.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Berberine Delays Onset of Collagen-Induced Arthritis through T Cell Suppression

doi: 10.3390/ijms22073522

Figure Lengend Snippet: The effect of berberine on circulating anti-bovine type II collagen (CII) IgG in the CIA model. ( A ) Anti-CII IgG1, IgG2a, and total IgG at day 28 among all mice (arthritic and non-arthritic) within BBR, PBS (vehicle control), CIA (no treatment control), and non-CIA control animals ( n = 10 per group). ( B ) Anti-CII IgG levels at day 28 compared among arthritic mice only (BBR n = 5; PBS n = 9; CIA n = 9). Statistical comparisons made with the Kruskal–Wallis test with Dunn’s multiple comparisons. ( C ) Anti-CII IgG levels at day 28 compared among BBR-treated mice who developed arthritis (“arthritic”) vs. those that did not (“non-arthritic”). Statistical comparisons made with the Mann–Whitney U test. For all statistical tests in A–C, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Serum concentrations of anti-collagen type II (anti-CII) total IgG (catalog # 1012T), anti-CII IgG1 (catalog # 20321T), and anti-CII IgG2a (catalog # 20322T) were measured by ELISA (Chondrex, Redmond, WA, USA) according to the manufacturer’s instructions [ , ].

Techniques: MANN-WHITNEY

Journal: Acta Pharmaceutica Sinica. B

Article Title: Augmentation of PRDX1–DOK3 interaction alleviates rheumatoid arthritis progression by suppressing plasma cell differentiation

doi: 10.1016/j.apsb.2025.06.006

Figure Lengend Snippet: PRDX1-OE attenuates the disease progression of RA by inhibiting plasma cell differentiation. (A) The clinical arthritis severity score was assessed in WT and Prdx1 -OE mice immunized with collagen in complete Freund's adjuvant ( n = 6). (B) Representative images of paws from the WT and PRDX1-OE group. (C) Representative micro-CT images of paws from the WT and PRDX1-OE groups. (D) Joint destruction from the WT and PRDX1-OE groups was graded using CT score ( n = 3). (E) Representative images of H&E-stained sections of ankle joints from the WT and PRDX1-OE groups are shown. Scale bar, 500 μm. (F) Histopathology analysis and histological scoring were performed on ankle joints from the WT and PRDX1-OE groups ( n = 6). (G) Serum levels of IgG2A were analyzed by ELISA in WT and PRDX1-OE model groups ( n = 6). (H) The proportion of plasma cells in the spleen was analyzed by flow cytometry. (I) A quantitative analysis of plasma cell counts in the spleens of Prdx1 -OE and wild-type mice ( n = 6). (J) GSEA enrichment profiles of the B cell receptor pathway and CD40 pathway in the spleens of Prdx1 -OE and WT model mice. Data are shown as mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: The serum was subjected to calculate the concentrations of IgG2A using the Mouse anti-bovine type II collagen IgG2A antibody subtype ELISA kit with TMB (Chondrex, 20322T).

Techniques: Biomarker Discovery, Clinical Proteomics, Cell Differentiation, Adjuvant, Micro-CT, Staining, Histopathology, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Acta Pharmaceutica Sinica. B

Article Title: Augmentation of PRDX1–DOK3 interaction alleviates rheumatoid arthritis progression by suppressing plasma cell differentiation

doi: 10.1016/j.apsb.2025.06.006

Figure Lengend Snippet: Salvianolic acid B prevents the progression of collagen-induced arthritis by enhancing the interaction between PRDX1 and DOK3. (A) Clinical scores of arthritis in mice treated with vehicle, 1.5 mg/kg TF, and 15 mg/kg or 30 mg/kg SAB, and immunized with collagen in complete Freund's adjuvant ( n = 6). (B) Representative micro-CT images of the hind paws from mice treated with vehicle, TF, and SAB. (C) Joint destruction was graded based on the CT score in B ( n = 3). (D, E) histological score of the inflammation area in mice treated with vehicle, TF, and SAB ( n = 6) (D) and representative images of hematoxylin and eosin (H&E)-stained paw sections (E). Scale bar, 100 μm. (F) Serum titer of IgG2A analyzed from vehicle, TF, and SAB by ELISA ( n = 6). (G, H) The frequency of plasma cells (G) and GC B cells (H) in the spleen from mice treated with vehicle, TF, and SAB was analyzed by flow cytometry ( n = 6). (I) GSEA enrichment profiles of the B cell receptor pathway and CD40 pathway in the SAB-H treatment and model groups. (J) Heatmap depicting the expression levels of the B cell receptor signaling pathway and RA defined by the differentially downregulated genes in the SAB-H treatment group compared to the WT model group. (K) The biological process analysis of B cells for downregulated genes in the SAB-H treatment group compared to the model group. (L) Representative immunofluorescence staining images of PRDX1 (green) and DOK3 (red) in the spleen from the model and SAB treatment groups. Scale bar, 50 μm. (M) Representative immunofluorescence staining images of CD19 (green), P-JNK (red) in the spleen from the model and SAB treatment groups. Scale bar, 50 μm. (N) Relative fluorescence intensity of stained PRDX1 and DOK3 in the spleen from the model and SAB treatment groups ( n = 3). (O) Relative fluorescence intensity of stained CD19 and P-JNK in the spleen from the model and SAB treatment groups ( n = 3). Data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, and ns indicates no significance.

Article Snippet: The serum was subjected to calculate the concentrations of IgG2A using the Mouse anti-bovine type II collagen IgG2A antibody subtype ELISA kit with TMB (Chondrex, 20322T).

Techniques: Adjuvant, Micro-CT, Staining, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Flow Cytometry, Expressing, Immunofluorescence, Fluorescence

Journal: Journal of Cellular and Molecular Medicine

Article Title: Activation of formyl peptide receptor 1 elicits therapeutic effects against collagen‐induced arthritis

doi: 10.1111/jcmm.16854

Figure Lengend Snippet: WKYMVm administration elicits beneficial effects against CIA. (A‐D) Vehicle or WKYMVm (4 mg/kg) was subcutaneously injected daily into CIA mice from secondary boosting at day 21. Mice were sacrificed on day 35. (A) Paw thickness (top) and clinical scores (bottom) of CIA mice were monitored during the indicated period. (B) Paw swelling of the two groups compared at the day of sacrifice. Representative images are shown. (C) CII reactive IgG1 and IgG2a were measured in peripheral blood serum from each group of mice. (D) Joint tissue sections were stained with H&E and safranin O (×40). Representative figures are shown. Data are expressed as the mean ± SEM ( n = 4–6 per group). p values were calculated by two‐way ANOVA (A) or Student's t test (C). * p < 0.05, *** p < 0.001

Article Snippet: The levels of IgG1 and IgG2a reactive to immunized collagen in the peripheral blood serum were determined by using a mouse anti‐bovine CII IgG1 and IgG2a antibody assay kit with tetramethylbenzidine (TMB) substrate (Chondrex, Redmond, WA, USA).

Techniques: Injection, Staining